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Three Different Routes of Inoculation for Experimental Infection with Schmallenberg Virus in Sheep
Author(s) -
Martinelle L.,
Poskin A.,
Dal Pozzo F.,
Mostin L.,
Van Campe W.,
Cay A. B.,
De Regge N.,
Saegerman C.
Publication year - 2017
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12356
Subject(s) - orthobunyavirus , inoculation , seroconversion , virology , biology , virus , spleen , viremia , immunization , veterinary medicine , medicine , immunology , immune system
Summary Schmallenberg virus ( SBV ) is an emerging Orthobunyavirus affecting European domestic ruminants. In this study, three groups of ewes ( n = 3) were inoculated with 1 ml of an SBV infectious serum, via the subcutaneous ( SC ), intradermal ( ID ) or intranasal ( IN ) route. The ewes were monitored for 10 days and no clinical signs were reported. IN inoculation failed to generate any detectable RNA emia. SC and ID inoculation induced typical SBV RNA emia and seroconversion upon day 6 post‐inoculation in 3/3 and 2/3 sheep, respectively. In all the animals that showed RNA emia, the viral genome could be detected in spleen and mesenteric lymph nodes. Both the SC and ID routes seem suitable to properly reproduce field conditions, as comparable observations were reported regarding RNA emia, seroconversion and viral genome detection in organs.