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Phylogenetic Analysis of Canine Parvovirus VP 2 Gene in C hina
Author(s) -
Yi L.,
Tong M.,
Cheng Y.,
Song W.,
Cheng S.
Publication year - 2016
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12268
Subject(s) - canine parvovirus , phylogenetic tree , biology , phylogenetics , gene , strain (injury) , virology , sequence analysis , genetics , parvovirus , virus , anatomy
Summary In this study, a total of 37 samples (58.0%) were found through PCR assay to be positive for canine parvovirus ( CPV ) of 66 suspected faecal samples of dogs collected from various cities throughout C hina. Eight CPV isolates could be obtained in the CRFK cell line. The sequencing of the VP 2 gene of CPV identified the predominant CPV strain as CPV ‐2a ( S er297 A la), with two CPV ‐2b ( S er297 A la). Sequence comparison revealed homologies of 99.3–99.9%, 99.9% and 99.3–99.7% within the CPV 2a isolates, within the CPV 2b isolates and between the CPV 2a and 2b isolates, respectively. In addition, several non‐synonymous and synonymous mutations were also recorded. The phylogenetic tree revealed that most of the CPV strains from different areas in C hina were located in the formation of a large branch, which were grouped together along with the KU 143‐09 strain from T hailand and followed the same evolution. In this study, we provide an updated molecular characterization of CPV 2 circulation in C hina.