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In vitro Culture of a Novel Genotype of E hrlichia sp. from B razil
Author(s) -
Zweygarth E.,
Schöl H.,
Lis K.,
Cabezas Cruz A.,
Thiel C.,
Silaghi C.,
Ribeiro M. F. B.,
Passos L. M. F.
Publication year - 2013
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12122
Subject(s) - ehrlichia , biology , anaplasmataceae , 16s ribosomal rna , ehrlichia canis , microbiology and biotechnology , canis , phylogenetic tree , ehrlichiosis , tick , virology , anaplasma , gene , genetics , antibody , botany , serology
Summary Ehrlichiae are obligate intracytoplasmic Gram‐negative, tick‐borne bacteria belonging to the Anaplasmataceae family. Ehrlichioses are considered emerging diseases in both humans and animals. Several members of the genus E hrlichia have been isolated and propagated in vitro . This study describes the continuous propagation of a B razilian E hrlichia sp. isolate in IDE8 tick cells, canine DH 82 cells and bovine aorta cells. Initially, the organisms were isolated from the haemolymph of a R hipicephalus ( B oophilus ) microplus tick into IDE 8 cells. Infected IDE 8 cells were brought from B razil to G ermany, where the organisms were continuously propagated in IDE 8, DH 82 and bovine aorta cells. Bovine aorta cells were infected and propagated for 3 months, corresponding to six subcultures, whereas the other two infected cell lines were kept for more than 1 year. During the cultivation period, 36 and 14 subcultures were carried out in IDE 8 and DH 82 cell cultures, respectively. Reinfection of IDE 8 cells with organisms grown in DH 82 cells was achieved. Sequence analysis made with a fragment of the 16S rRNA gene showed that this E hrlicha sp. is closely related to E hrlichia canis . However, the maximum likelihood phylogenetic tree shows that it falls in a separate phylogenetic clade from E . canis .