Premium
Isolation and Genetic Characterization of a Tembusu Virus Strain Isolated From Mosquitoes in Shandong, China
Author(s) -
Tang Y.,
Diao Y.,
Chen H.,
Ou Q.,
Liu X.,
Gao X.,
Yu C.,
Wang L.
Publication year - 2015
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12111
Subject(s) - biology , flavivirus , virology , virus , genetics , phylogenetic tree , gene
Summary Tembusu virus ( TMUV ) is a flavivirus, presumed to be a mosquito‐borne flavivirus of the Ntaya virus subgroup. To date, however, there have been no reports indicating that mosquitoes are involved in the spread of TMUV . In this study, we report the first isolation of TMUV from Culex mosquitoes. We describe the isolation and characterization of a field strain of TMUV from mosquitoes collected in Shandong Province, China. The virus isolate, named TMUV ‐ SDMS , grows well in mosquito cell line C6/36, in Vero and duck embryo fibroblast ( DEF ) cell lines, and causes significant cytopathic effects in these cell cultures. The TMUV ‐ SDMS genome is a single‐stranded RNA , 10 989 nt in length, consisting of a single open reading frame encoding a polyprotein of 3410 amino acids, with 5′ and 3′ untranslated regions of 142 and 617 nt, respectively. Phylogenetic analysis of the E and NS 5 genes revealed that the TMUV ‐ SDMS is closely related to the TMUV YY 5 and BYD strains which cause severe egg‐drop in ducks. The 3′ NTR of TMUV ‐ SDMS contains two pairs of tandem repeat CS and one non‐duplicate CS , which have sequence similarities to the same repeats in the YY 5 and BYD strains. Our findings indicate that mosquitoes carrying the TMUV may play an important role in the spread of this virus and in disease outbreak.