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Biological Control of A eromonas salmonicida subsp. salmonicida Infection in Rainbow Trout ( O ncorhynchus mykiss ) Using A eromonas Phage PAS ‐1
Author(s) -
Kim J. H.,
Choresca C. H.,
Shin S. P.,
Han J. E.,
Jun J. W.,
Park S. C.
Publication year - 2015
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12088
Subject(s) - aeromonas salmonicida , rainbow trout , microbiology and biotechnology , biology , aeromonas , clearance , bacteria , phage therapy , trout , bacteriophage , fish <actinopterygii> , fishery , gene , medicine , escherichia coli , genetics , urology , biochemistry
Summary The potential control efficacy of A eromonas phage PAS ‐1 was evaluated against A eromonas salmonicida subsp. salmonicida infection in rainbow trout ( O ncorhynchus mykiss ) model in this study. The phage was co‐cultured with the virulent A . salmonicida subsp. salmonicida strain AS 05 that possesses the type III secretion system ( TTSS ) ascV gene, and efficient bacteriolytic activity was observed against the bacteria. The administration of PAS ‐1 in rainbow trout demonstrated that the phage was cleared from the fish within 200 h post‐administration, and a temporal neutralizing activity against the phage was detected in the sera of phage‐administrated fish. The administration of PAS ‐1 (multiplicity of infection: 10 000) in A . salmonicida subsp. salmonicida infected rainbow trout model showed notable protective effects, with increased survival rates and mean times to death. These results demonstrated that A eromonas phage PAS ‐1 could be considered as an alternative biological control agent against A . salmonicida subsp. salmonicida infections in rainbow trout culture.