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Phylogenetic and Phylogeographic Analyses of P orcine Circovirus Type 2 Among Pig Farms in V ietnam
Author(s) -
Huynh T. M. L.,
Nguyen B. H.,
Nguyen V. G.,
Dang H. A.,
Mai T. N.,
Tran T. H. G.,
Ngo M. H.,
Le V. T.,
Vu T. N.,
Ta T. K. C.,
Vo V. H.,
Kim H. K.,
Park B. K.
Publication year - 2014
Publication title -
transboundary and emerging diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.392
H-Index - 63
eISSN - 1865-1682
pISSN - 1865-1674
DOI - 10.1111/tbed.12066
Subject(s) - porcine circovirus , capsid , biology , virology , phylogenetic tree , circovirus , recombinant dna , genome , genetics , phylogenetics , virus , gene
Summary This study demonstrated the prevalence of Porcine circovirus type 2 ( PCV 2) among pig farms in V ietnam. Analyses of the genome, capsid protein and phylogeny classified all 30 V ietnamese PCV 2 strains as the PCV 2b genotype, belonging to the clusters of 1 A , 1 B , 1 C and recombinant forms. Each viral genome was 1767 nucleotides long and shared 96.0–100% nucleotide sequence identity. The amino acid substitutions in the capsid protein of the V ietnamese PCV 2 strains were in immunodominant regions, and the majority of strains (24/30) contained a lysine extension at the C ‐terminus. Bayesian phylogeographic analysis revealed epidemic links of the PCV 2 recombinant cluster within and among countries, which supports a circulating recombinant form of PCV 2. Further analysis by the J ameson– W olf antigenic index indicated antigenic alterations at important sites in the capsid protein (sites 131–133) among the recombinant cluster and the other clusters of PCV 2b.