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HLA‐B *27 typing using a triplex real time PCR in routine laboratory
Author(s) -
Viken Marte K.,
Pedersen Alice L.,
Andersen Menaka,
Jensen Tore,
Lie Benedicte A.,
Boulland Line M. L.
Publication year - 2021
Publication title -
hla
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.347
H-Index - 99
eISSN - 2059-2310
pISSN - 2059-2302
DOI - 10.1111/tan.14386
Subject(s) - typing , multiplex , human leukocyte antigen , real time polymerase chain reaction , computational biology , biology , microbiology and biotechnology , genetics , antigen , gene
We have established an in‐house HLA‐B*27 multiplex typing assay by using a combination of previously published, newly designed and commercial primers and probes for use with real‐time PCR instruments. Hence, facilitating quick and large‐scale HLA‐B*27 typing.