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Extending the sequences of HLA class I alleles without full‐length genomic coverage using single molecule real‐time DNA sequencing
Author(s) -
Hassall Kylara B.,
Latham Katy,
Robinson James,
Gymer Arthur,
Goodall Rebecca,
Merlo Dario,
Marsh Steven G. E.,
Mayor Neema P.
Publication year - 2020
Publication title -
hla
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.347
H-Index - 99
eISSN - 2059-2310
pISSN - 2059-2302
DOI - 10.1111/tan.13800
Subject(s) - human leukocyte antigen , genetics , sequence (biology) , allele , biology , dna sequencing , computational biology , genomic dna , typing , reference genome , sequence analysis , dna , gene , antigen
The assignment of an HLA allele name to a sequence requires a comparison between the generated target sequence and a reference sequence on the IPD‐IMGT/HLA database. Absence of a full‐length reference sequence can result in the inability of HLA typing software to accurately compare and assign the sequence. We sequenced the most frequently seen HLA class I alleles on the Anthony Nolan register present in the database with only a partial genomic sequence, with the aim of increasing the number of complete reference sequences. We successfully extended 95 full‐length HLA class I sequences and identified 13 novel variants. Increasing the number of full‐length HLA class I reference sequences in the database has aided accuracy of HLA analysis tools for all histocompatibility and immunogenetics laboratories.