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Quantitative polymerase chain reaction technology in chimerism monitoring after hematopoietic stem cell transplantation: One center experience
Author(s) -
Stingl Jankovic Katarina,
Maskalan Marija,
Burek Kamenaric Marija,
Zunec Renata,
Durakovic Nadira,
ServentiSeiwerth Ranka,
Vrhovac Radovan,
Grubic Zorana
Publication year - 2019
Publication title -
hla
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.347
H-Index - 99
eISSN - 2059-2310
pISSN - 2059-2302
DOI - 10.1111/tan.13707
Subject(s) - hematopoietic stem cell transplantation , polymerase chain reaction , microchimerism , transplantation , stem cell , immunology , population , medicine , biology , oncology , genetics , pregnancy , fetus , environmental health , gene
Chimerism status evaluation is a routine test performed in post‐hematopoietic stem cell transplantation (HSCT) period. The aim of the study was to evaluate a quantitative polymerase chain reaction (qPCR) method (GenDx, Utrecht, the Netherlands) applicability for this purpose. The study included 74 recipient/donor pairs tested for informative markers: median of four and six informative markers was found for patients (related and unrelated donor, respectively). Higher sensitivity of qPCR method was confirmed by analysis of recipient post‐HSCT samples (N = 800) among which microchimerism (0.1%‐1% recipient DNA) was detected in 21.8% of cases. The ability to detect less than 1% of minor population, as opposed to the short tandem repeat (STR) method for which 1% is the limit, translated into earlier identification of a disease relapse for four patients in our study sample.