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A novel antibody combination to identify KIR2DS2 high natural killer cells in KIR2DL3/L2/S2 heterozygous donors
Author(s) -
Blunt Matthew D.,
Rettman Pauline,
BastidasLegarda Leidy Y.,
Fulton Rebecca,
Capizzuto Valentina,
Naiyer Mohammed M.,
Traherne James A.,
Khakoo Salim I.
Publication year - 2019
Publication title -
hla
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.347
H-Index - 99
eISSN - 2059-2310
pISSN - 2059-2302
DOI - 10.1111/tan.13413
Subject(s) - antibody , transfection , natural killer cell , cell , cell culture , computational biology , biology , immunology , cytotoxicity , in vitro , genetics
The killer cell immunoglobulin‐like receptor (KIR) KIR2DS2 induces natural killer (NK) cell activation upon ligation and in genetic studies is associated with protection against certain cancers and viral infections. One of the difficulties in understanding KIR2DS2 has been that ligands have been hard to define. In part, this is because the high sequence homology between KIR2DS2 and KIR2DL3/KIR2DL2 has made it difficult to make antibodies that specifically detect NK cells expressing KIR2DS2. Using transfected NK cell line (NKL) cells and primary human samples, we report the identification of a novel antibody combination which allows identification of NK cells with relatively high expression of KIR2DS2. This separation is sufficient to examine primary human NK cell activation in response to KIR2DS2 specific ligands.