Open AccessControlled mechanical vibration and impacts on skin biology
Author(s) -
Caberlotto Elisa,
Bernal Miguel,
Miller Zane,
Poole Aaron,
Ruiz Laetitia,
Tanter Mickaël,
Gennisson JeanLuc,
Querleux Bernard
Publication year - 2019
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/srt.12753
Subject(s) - stimulation , explant culture , human skin , biomedical engineering , ex vivo , in vivo , in vitro , ultrasound , effector , biophysics , microbiology and biotechnology , materials science , chemistry , biology , medicine , neuroscience , biochemistry , genetics , radiology
Background and objective Different biological models have shown how mechanical stimulation may induce physiological responses from solicited cells, tissues, or organs. In models of cultured skin cells, the frequency of the mechanical stress appears to be a paramount parameter, generating a biological response in some cells, particularly from dermal fibroblasts. Our objective was to explore in ex vivo human skin explants the effects of mechanical stimulation. Materials and methods Mechanical stimulations were provided by a torque test device, with different end effectors, able to generate cyclic strains at different frequencies (from 40 to 120 Hz). Skin explant samples were stimulated twice daily by the device for one minute, over 10 days. Results At days 0, 5, and 10, samples were processed by immunohistological procedures, allowing some structural dermal proteins to be quantified (fluorescence). As compared to untreated skin explant samples, the stimulation procedure clearly led some proteins of the dermal‐epidermal and some dermal proteins to be overexpressed. This stimulation was found to be frequency‐dependent, with the greatest overall increases occurring at 60 and 90 Hz. Conclusion For the first time, ultrafast ultrasound imaging in vitro (phantom mimicking skin mechanical properties) was used to analyze mechanical waves transmitted to the skin layers as a function of end effector shape.