Open AccessAnesthesia effects on the low frequency blood flow oscillations in mouse skin
Author(s) -
Astashev M. E.,
Serov D. A.,
Tankanag A. V.
Publication year - 2019
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/srt.12593
Subject(s) - xylazine , anesthesia , blood flow , nitrous oxide , isoflurane , laser doppler velocimetry , perfusion , chemistry , medicine , ketamine
Background When laboratory animals are used one needs to anesthetize them before recording. However, the influence of anesthesia on animal blood flow oscillations has not been studied. The effects of two ways of anesthesia, zoletil‐xylazine, and zoletil‐nitrous oxide mixtures, on mouse skin perfusion using laser Doppler flowmetry ( LDF ) technique were studied. Methods BALB /c mice were used. LDF probe was placed on the ventral surface of the left hind paw. Spectral analysis of LDF signals was performed with continuous adaptive wavelet transform to identify and describe peripheral blood flow oscillations in mouse skin. Results Low‐frequency oscillation interval boundaries (myogenic, neurogenic, and endothelial) for mice were shown to coincide with the boundaries determined for human and rats, that demonstrate their independence from the body size. Zoletil‐xylazine anesthesia significantly decreased neurogenic and endothelial oscillation amplitudes by 29% and 50% respectively and increased the amplitude of cardiac oscillations by 23% compared to zoletyl‐nitrous oxide anesthesia. There were no significant changes of the amplitudes of myogenic and respiratory oscillations with zoletil‐nitrous oxide anesthesia compared to the zoletil‐xylazine mixture. Conclusion We suggest that the different influence of anesthesia modes on the amplitudes of skin blood flow oscillations is associated with sympathetic activity suppressed by zoletil‐xylazine anesthesia.