Open AccessOptical coherence tomography: An efficient imaging method for the visualization of human epidermis orientation
Author(s) -
Mias C.,
Filiol J.,
Josse G.
Publication year - 2018
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/srt.12414
Subject(s) - optical coherence tomography , epidermis (zoology) , orientation (vector space) , biomedical engineering , context (archaeology) , microscopy , visualization , suction blister , blisters , materials science , optics , computer vision , computer science , artificial intelligence , medicine , anatomy , biology , physics , mathematics , paleontology , geometry , composite material
Recently, it has been shown that epidermal sheets taken from suction blisters are very appropriate skin samples for Multi‐Photon ( MP ) microscopy. However, we observed that image quality was much better when the sample was visualized through the basale side. Thus, the epidermis orientation needs to be controlled before MP imaging. We observed that the use of standard laboratory binoculars led to a high rate of false results. In this context, we showed that optical coherence tomography provided clear images of the epidermis orientation without loss of sample integrity and thus represents an effective technique before slide sealing and MP analysis.