Open AccessAutofluorescence of collagen fibres in scar
Author(s) -
Zhao H. L.,
Zhang C. P.,
Zhu H.,
Jiang Y. F.,
Fu X. B.
Publication year - 2017
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/srt.12375
Subject(s) - autofluorescence , dermis , reticular dermis , pathology , epidermis (zoology) , scar tissue , chemistry , anatomy , fluorescence , medicine , optics , surgery , physics
Background/purpose Autofluorescence has become an important factor associated with diagnosis and treatment of many diseases. Methods Full thickness skin grafts and scar biopsies were obtained from five volunteers. The normal skin or scar tissue paraffin‐wax sections were stained with HE and the autofluorescence of collagen fibres was viewed under fluorescence microscopy. Results In normal skin, the autofluorescence was showed in dermis, specifically in collagen fibres. There was very weak autofluorescence in epidermis. The spectrum was excited at 488 nm and the peak value of autofluorescence was significantly different between reticular layer (169.24±9.18) and papillar layer of dermis (103.91±15.23). In scar tissue, the autofluorescence was showed in collagen fibres and the peak value was 176.71±20.69. The structure of collagen fibres in normal skin or scar tissue was different in loose degree, thickness, boundle size, and morphology by their autofluorescence. Conclusion The different peak value of autofluorescence between scar and normal skin may due to the different density of collagen fibtes in them. This study may provide us a simple and effective assessment indicator and method for diagnosis and treatment of scar.