Open AccessTranscutaneous pneumatic injection of glucose solution: a morphometric evaluation of in vivo micropig skin and tissue‐mimicking phantom
Author(s) -
Cho S. B.,
Kwon T. R.,
Yoo K. H.,
Oh C. T.,
Choi E. J.,
Kim B. J.
Publication year - 2017
Publication title -
skin research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.521
H-Index - 69
eISSN - 1600-0846
pISSN - 0909-752X
DOI - 10.1111/srt.12306
Subject(s) - dermis , biomedical engineering , in vivo , subcutaneous tissue , imaging phantom , transdermal , infiltration (hvac) , penetration (warfare) , wound healing , hypodermic needle , tissue engineering , anatomy , medicine , materials science , pathology , surgery , nuclear medicine , syringe , biology , pharmacology , microbiology and biotechnology , composite material , operations research , engineering , psychiatry
Background Needle‐free, transcutaneous pneumatic injection systems can be used to deliver therapeutic solutions to targeted layers of skin in a minimally invasive manner. Methods To evaluate jet infiltration patterns and tissue reactions, 5% isotonic and 20% hypertonic glucose solutions were pneumatically injected into in vivo micropig skin. Gelatin TM phantom was additionally prepared to analyze penetration and dispersion patterns for different experimental settings. Results As immediate tissue reactions in the in vivo micropig skin, distinct pneumatic injection injury zones ( PIIZ s) in the dermis, extending from the papillary dermis deep into the dermo‐subcutaneous junction, were generated with the 5% and 20% glucose solutions and with pneumatic pressures of 4.64 and 5.7 bars, respectively. PIIZ s markedly decreased in appearance at 1 day after treatment, accompanied by inflammatory cell infiltration, and disappeared at 7 days post‐treatment with increased collagen and elastin production. In TM phantom study, the PIIZ s created by 20% glucose mainly comprised a single, homogenous, round to oval zone, whereas those created by 5% glucose were irregular and multi‐lobular. Conclusion The present study suggests that transcutaneous pneumatic injection therapy may exert mechanical stimulatory effects, immediate tissue shrinkage via hypertonic solutions, and late tissue regeneration effects during wound healing.