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Exercise dose affects the circulating microRNA profile in response to acute endurance exercise in male amateur runners
Author(s) -
FernándezSanjurjo Manuel,
Úbeda Natalia,
FernándezGarcía Benjamín,
del Valle Miguel,
Ramírez de Molina Ana,
Crespo María Carmen,
MartínHernández Roberto,
CasasAgustench Patricia,
MartínezCamblor Pablo,
de GonzaloCalvo David,
DíezRobles Sergio,
GarcíaGonzález Ángela,
Montero Ana,
GonzálezGonzález Felipe,
Rabadán Manuel,
DíazMartínez Ángel Enrique,
Whitham Martin,
IglesiasGutiérrez Eduardo,
Dávalos Alberto
Publication year - 2020
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/sms.13759
Subject(s) - microrna , context (archaeology) , endurance training , downregulation and upregulation , biomarker , gene expression , in silico , medicine , bioinformatics , biology , endocrinology , gene , genetics , paleontology
The systemic response to exercise is dose‐dependent and involves a complex gene expression regulation and cross‐talk between tissues. This context ARISES the need for analyzing the influence of exercise dose on the profile of circulating microRNAs (c‐miRNAs), as emerging posttranscriptional regulators and intercellular communicators. Thus, we hypothesized that different exercise doses will determine specific c‐miRNA signatures that will highlight its potential as exercise dose biomarker. Nine active middle‐aged males completed a 10‐km race (10K), a half‐marathon (HM), and a marathon (M). Blood samples were collected immediately before and after races. Plasma RNA was extracted, and a global screening of 752 microRNAs was analyzed using RT‐qPCR. Three different c‐miRNA profiles were defined according to the three doses. In 10K, 14 c‐miRNAs were found to be differentially expressed between pre‐ and post‐exercise, 13 upregulated and 1 downregulated. Regarding HM, 13 c‐miRNAs were found to be differentially modulated, in all the cases upregulated. A total of 28 c‐miRNAs were found to be differentially expressed in M, 21 overexpressed and 7 repressed after this race. We had also found 3 common c‐miRNAs between 10K and M and 2 common c‐miRNAs between 10K and HM. In silico analysis supported a close association between exercise dose c‐miRNA profiles and cellular pathways linked to energy metabolism and cell cycle. In conclusion, we have observed that different exercise doses induced specific c‐miRNA profiles. So, our results point to c‐miRNAs as emerging exercise dose biomarkers and as one of regulatory mechanisms modulating the response to endurance exercise.