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Lipoprotein lipase activity does not predict very low‐density lipoprotein‐triglyceride fatty acid oxidation during exercise
Author(s) -
Søndergaard E.,
Andersen I. R.,
Sørensen L. P.,
Gormsen L. C.,
Nielsen S.
Publication year - 2017
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/sms.12859
Subject(s) - lipoprotein lipase , medicine , endocrinology , very low density lipoprotein , adipose tissue , triglyceride , chemistry , lipoprotein , cholesterol
Exercise lowers plasma triglyceride levels, but the physiological mechanisms remain not fully elucidated. Lipoprotein lipase ( LPL ) is a key enzyme in facilitating fatty acid uptake from lipoproteins. As exercise increases the efficiency of very low‐density lipoprotein‐triglyceride ( VLDL ‐ TG ) oxidation, we hypothesized that muscle LPL activity would be a rate‐limiting step and predict VLDL ‐ TG Fatty acids oxidation during exercise. Sixteen healthy, lean subjects (eight men and eight women) were examined before and during an acute exercise bout (90 minutes at 50% of VO 2‐max). Heparin‐releasable LPL activity was measured in muscle and adipose tissue biopsies. Breath 14 CO 2 was measured after a primed‐constant infusion of ex vivo labeled [ 14 C]‐triolein VLDL ‐ TG . Fractional VLDL ‐ TG storage was measured in adipose tissue biopsies. Exercise did not affect muscle LPL activity ( P =.30). No association was observed between muscle LPL activity and VLDL ‐ TG oxidation, neither in the basal state ( P =.17) nor during exercise ( P =.83). Exercise did not affect upper body or lower body adipose tissue LPL activity (both P =.92). The basal adipose tissue fractional VLDL ‐ TG storage (abdominal.13%±9%; femoral 17%±10% ( P =.18)) was not associated with upper body ( P =.56) or lower body ( P =.44) subcutaneous adipose tissue LPL activity. Muscle LPL activity does not predict VLDL ‐ TG oxidation during rest or exercise. In addition, adipose tissue LPL activity was not associated with VLDL ‐ TG storage during rest. This suggests that LPL activity is present in excess of what is required to facilitate lipid uptake for oxidation during both rest and exercise.