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Extracellular and cellular Hsp72 differ as biomarkers in acute exercise/environmental stress and recovery
Author(s) -
Lee E. CH.,
Muñoz C. X.,
McDermott B. P.,
Beasley K. N.,
Yamamoto L. M.,
Hom L. L.,
Casa D. J.,
Armstrong L. E.,
Kraemer W. J.,
Anderson J. M.,
Maresh C. M.
Publication year - 2017
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/sms.12621
Subject(s) - biomarker , heat illness , medicine , extracellular , dehydration , extracellular fluid , heat stress , thermoregulation , hyperthermia , urine , endocrinology , zoology , chemistry , biochemistry , biology , physics , meteorology
Stress‐inducible Hsp72 is a potential biomarker to track risk of exertional heat illness during exercise/environmental stress. Characterization of extracellular ( eH sp72) vs cellular Hsp72 ( iH sp72) responses is required to define the appropriate use of Hsp72 as a reliable biomarker. In each of four repeat visits, participants ( n = 6 men, 4 trials; total n = 24): (a) passively dehydrated overnight, (b) exercised (2 h) with no fluid in a hot, humid environmental chamber, (c) rested and rehydrated (1 h), (d) maximally exercised for 0.5 h, and (e) returned after 24 h of at‐home recovery and rehydration. We measured rectal temperature, hydration status (% body mass loss, urine markers, serum osmolality), and Hsp72 ( ELISA , flow cytometry. eH sp72 (circulating) and iH sp72 ( CD 3 + PBMC s) correlated ( P < 0.05) with markers of heat, exercise, and dehydration stresses. eH sp72 immediately post‐exercise (>15% above baseline, P < 0.05) decreased back to baseline levels by 1 h post‐exercise, but iH sp72 expression continued to rise and remained elevated 24 h post‐exercise (~2.5‐fold baseline, P < 0.05). These data suggest that in addition to the classic physiological biomarkers of exercise heat stress, using cellular Hsp72 as an indicator of lasting effects of stress into recovery may be most appropriate for determining long‐term effects of stress on risk for exertional heat illness.