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Repeated high‐intensity exercise modulates Ca 2+ sensitivity of human skeletal muscle fibers
Author(s) -
Gejl K. D.,
Hvid L. G.,
Willis S. J.,
Andersson E.,
Holmberg H.C.,
Jensen R.,
Frandsen U.,
Hansen J.,
Plomgaard P.,
Ørtenblad N.
Publication year - 2016
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/sms.12483
Subject(s) - chemistry , dithiothreitol , skeletal muscle , medicine , exercise intensity , endocrinology , fiber , biophysics , biology , biochemistry , blood pressure , heart rate , organic chemistry , enzyme
The effects of short‐term high‐intensity exercise on single fiber contractile function in humans are unknown. Therefore, the purposes of this study were: (a) to access the acute effects of repeated high‐intensity exercise on human single muscle fiber contractile function; and (b) to examine whether contractile function was affected by alterations in the redox balance. Eleven elite cross‐country skiers performed four maximal bouts of 1300 m treadmill skiing with 45 min recovery. Contractile function of chemically skinned single fibers from triceps brachii was examined before the first and following the fourth sprint with respect to Ca 2+ sensitivity and maximal Ca 2+ ‐activated force. To investigate the oxidative effects of exercise on single fiber contractile function, a subset of fibers was incubated with dithiothreitol ( DTT ) before analysis. Ca 2+ sensitivity was enhanced by exercise in both MHC I (17%, P < 0.05) and MHC II (15%, P < 0.05) fibers. This potentiation was not present after incubation of fibers with DTT . Specific force of both MHC I and MHC II fibers was unaffected by exercise. In conclusion, repeated high‐intensity exercise increased Ca 2+ sensitivity in both MHC I and MHC II fibers. This effect was not observed in a reducing environment indicative of an exercise‐induced oxidation of the human contractile apparatus.