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Oral quercetin supplementation hampers skeletal muscle adaptations in response to exercise training
Author(s) -
Casuso R. A.,
MartínezLópez E. J.,
Nordsborg N. B.,
HitaContreras F.,
MartínezRomero R.,
Cañuelo A.,
MartínezAmat A.
Publication year - 2014
Publication title -
scandinavian journal of medicine and science in sports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.575
H-Index - 115
eISSN - 1600-0838
pISSN - 0905-7188
DOI - 10.1111/sms.12136
Subject(s) - skeletal muscle , medicine , physical therapy , physical medicine and rehabilitation , quercetin , exercise physiology , biology , antioxidant , biochemistry
We aimed to test exercise‐induced adaptations on skeletal muscle when quercetin is supplemented. Four groups of rats were tested: quercetin sedentary, quercetin exercised, placebo sedentary, and placebo exercised. Treadmill exercise training took place 5 days a week for 6 weeks. Quercetin groups were supplemented with quercetin, via gavage, on alternate days throughout the experimental period. Sirtuin 1 ( SIRT1 ), peroxisome proliferator‐activated receptor γ coactivator‐1α mRNA levels, mitochondrial DNA ( mtDNA ) content, and citrate synthase ( CS ) activity were measured on quadriceps muscle. Redox status was also quantified by measuring muscle antioxidant enzymatic activity and oxidative damage product, such as protein carbonyl content ( PCC ). Quercetin supplementation increased oxidative damage in both exercised and sedentary rats by inducing higher amounts of PCC ( P < 0.001). Quercetin supplementation caused higher catalase ( P < 0.001) and superoxide dismutase ( P < 0.05) activity in the non‐exercised animals, but not when quercetin is supplemented during exercise. Quercetin supplementation increased SIRT1 expression, but when quercetin is supplemented during exercise, this effect is abolished ( P < 0.001). The combination of exercise and quercetin supplementation caused lower ( P < 0.05) mtDNA content and CS activity when compared with exercise alone. Quercetin supplementation during exercise provides a disadvantage to exercise‐induced muscle adaptations.