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C‐type lectin‐like CD161 is not a co‐signalling receptor in gluten‐reactive CD4 + T cells
Author(s) -
Wyrożemski Łukasz,
Sollid Ludvig M.,
Qiao ShuoWang
Publication year - 2021
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.13016
Subject(s) - t cell receptor , t cell , context (archaeology) , cd8 , biology , receptor , microbiology and biotechnology , chemistry , immunology , antigen , immune system , biochemistry , paleontology
Abstract C‐type lectin‐like CD161, a class II transmembrane protein, is a surface receptor expressed by NK cells and T cells. In coeliac disease, CD161 was expressed more frequently on gluten‐reactive CD4 + T cells compared to other memory CD4 + T cells isolated from the same tissue compartment. CD161 is a putative co‐signalling molecule that was proposed to act as co‐stimulatory receptor in the context of signalling through TCR, but contradicting results were published. In order to understand the role of CD161 in gluten‐reactive CD4 + T cells, we combined T cell stimulation assays or T cell proliferation assays with ligation of CD161 and intracellular cytokine staining. We found that CD161 ligation provided neither co‐stimulatory nor co‐inhibitory signals to modulate proliferation and IFN‐γ or IL‐21 production by gluten‐reactive CD4 + T cell clones. Thus, we suggest that CD161 does not function as a co‐signalling receptor in the context of gluten‐reactive CD4 + T cells.