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Monoclonal antibody therapy that targets phospholipid‐binding protein delays lupus activity in MRL/lpr mice
Author(s) -
Mihaylova Nikolina,
Bradyanova Silviya,
Chipinski Petroslav,
Chausheva Stela,
Kyurkchiev Dobroslav,
Tchorbanov Andrey I.
Publication year - 2020
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12915
Subject(s) - monoclonal antibody , autoantibody , antibody , systemic lupus erythematosus , autoimmunity , autoimmune disease , annexin a2 , immunology , annexin , flow cytometry , antigen , secretion , immunofluorescence , monoclonal , biology , microbiology and biotechnology , medicine , endocrinology , disease
Abstract Systemic lupus erythematosus is an autoimmune syndrome characterized by the development of autoantibodies to a wide range of antigens. Together with B cells, respective self‐reactive T cells have an important contribution in disease progression as being responsible for inflammatory cytokines secretion, B cell activation and promoting amplification of the autoimmune response. Annexin A1 is expressed by many cell types and binds to phospholipids in a Ca 2+ ‐dependent manner. Abnormal expression of annexin A1 was found on activated B and T cells in both murine and human autoimmunity suggesting its potential role as a therapeutic target. In the present study, we have investigated the possibility to suppress autoimmune manifestation in spontaneous mouse model of lupus using anti‐annexin A1 antibody. Groups of lupus‐prone MRL/lpr mice were treated with the anti‐annexin A1 monoclonal antibody, and the disease activity and survival of the animals were following up. Flow cytometry, ELISA assays, and histological and immunofluorescence kidney analyses were used to determine the levels of Annexin A1 expression, cytokines, anti‐dsDNA antibodies and kidney injuries. The administration of this monoclonal antibody to MRL/lpr mice resulted in suppression of IgG anti‐dsDNA antibody production, modulated IL‐10 secretion, decreased disease activity and prolonged survival compared with the control group.