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Transcription factor c‐Maf is essential for IL ‐10 gene expression in B cells
Author(s) -
Liu Min,
Zhao Xiaoqi,
Ma Yunfeng,
Zhou Yan,
Deng Ming,
Ma Yonggang
Publication year - 2018
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12701
Subject(s) - transcription factor , biology , regulatory b cells , interleukin 10 , immune system , microbiology and biotechnology , downregulation and upregulation , cytokine , gene expression , regulation of gene expression , transcriptional regulation , gene , immunology , genetics
Interleukin ( IL )‐10 is an essential anti‐inflammatory cytokine that plays important roles as a negative regulator of immune responses to microbial antigens. c‐Maf has been suggested as an essential transcriptional factor for IL ‐10 production in CD 4 + T cells and macrophages. However, it remains unclear whether c‐Maf participates in IL ‐10 expression in B cells. In this study, we investigated the role of c‐Maf in the transcriptional regulation of IL ‐10 in regulatory B cells, as well as the underlying molecular mechanism. We found that c‐Maf was constitutively expressed in resting B cells. c‐Maf expression was upregulated in the presence of LPS and dose‐dependently enhanced IL ‐10 production following binding to the IL ‐10 promoter. Moreover, a lower expression of c‐Maf and decreased production of regulatory B (Breg) cells were detected in mice with collagen‐induced arthritis ( CIA ), which may contribute to the pathological changes. Taken together, these data demonstrate that c‐Maf is an indispensable yet constitutive transcription factor for IL ‐10 gene expression in LPS ‐activated B cells.