Premium
Tspan33 is Expressed in Transitional and Memory B Cells, but is not Responsible for High ADAM 10 Expression
Author(s) -
PérezMartínez C. A.,
MaravillasMontero J. L.,
MezaHerrera I.,
VencesCatalán F.,
Zlotnik A.,
SantosArgumedo L.
Publication year - 2017
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12559
Subject(s) - subfamily , microbiology and biotechnology , transmembrane protein , adam10 , biology , flow cytometry , cell , gene , genetics , receptor , matrix metalloproteinase , metalloproteinase , disintegrin
Tetraspanins are a family of transmembrane proteins that form membrane microdomains. They play important roles in migration, adhesion and other cellular processes. TspanC8, a subfamily of tetraspanins, was found to associate and promote ADAM 10 trafficking and cell surface localization. One of its members, Tspan33, is expressed in activated B cells. Using RT ‐ PCR and flow cytometry, we analysed the pattern of expression of Tspan33 in B cells from healthy donors. We found Tspan33 expression in early and late stages of B cell development. However, Tspan33 expression did not correlate with ADAM 10 surface expression. We also found expression of Tspan33 early in the activation process. Given its predominant expression in activated B cells and in several lymphomas, but not in naive B cells, we hypothesize that Tspan33 could be a potential target for therapeutic purposes.