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Experimental Arthritis in the Rat Induced by the Superantigen Staphylococcal Enterotoxin A
Author(s) -
Gerlach K.,
Tomuschat C.,
Finke R.,
Staege M. S.,
Brütting C.,
Brandt J.,
Jordan B.,
Schwesig R.,
Rosemeier A.,
Delank K.S.,
Kornhuber M. E.,
Emmer A.
Publication year - 2017
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12530
Subject(s) - superantigen , enterotoxin , arthritis , immunology , microbiology and biotechnology , medicine , biology , genetics , t cell , gene , escherichia coli , immune system
The pathogenesis of rheumatoid arthritis ( RA ) is incompletely understood. Human endogenous retroviruses ( HERV s) and their superantigenic envelope protein (env) have been implicated in the pathogenesis of RA . In the present investigation, the arthritogenic potential of the superantigen staphylococcal enterotoxin A ( SEA ) has been investigated. In the present investigation, the bacterial superantigen staphylococcal enterotoxin A ( SEA ) was injected into the right knee joint of 15 Lewis rats. Further nine animals received saline. Animals were sacrificed one, five and 10 days after the injection, respectively. The antigens CD 3, CD 4, CD 8, MHC class I, MHC class II , Pax5 and CD 138 were investigated by immunohistochemistry on cryo‐sections. After intra‐articular SEA injection, the inflammation was initially dominated by CD 8+ T cells. In the course of the investigation, the numbers of CD 4+, Pax5+, CD 138+ and MHC class II + cells increased. CD 3 was expressed in low numbers as compared to CD 8. After saline injection, no similar inflammatory response has been detected. The arthritis induced by the superantigen SEA may be a novel model for inflammatory joint diseases, that is rheumatoid arthritis or juvenile idiopathic arthritis.