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C‐type Lectin Receptor Expression on Human Basophils and Effects of Allergen‐Specific Immunotherapy
Author(s) -
Lundberg K.,
Rydnert F.,
Broos S.,
Andersson M.,
Greiff L.,
Lindstedt M.
Publication year - 2016
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12457
Subject(s) - immunology , allergen , receptor , immune system , immunoglobulin e , flow cytometry , c type lectin , allergy , lectin , antigen , biology , antibody , biochemistry
Basophils are emerging as immunoregulatory cells capable of interacting with their environment not only via their characteristic IgE‐mediated activation, but also in an IgE‐independent manner. Basophils are known to express and respond to stimulation via TLR 2, TLR 4, DC ‐ SIGN and DCIR , but whether basophils also express other C‐type lectin receptors ( CLR s) is largely unknown. In this study, we investigate the CLR expression profile of human basophils using multicolour flow cytometry. As FcRs as well as some CLR s are associated with allergen recognition and shown to be involved in subsequent immune responses, the expression of CLR s and FcRs on peripheral blood basophils, as well as their frequency, was monitored for 1 year in subjects undergoing subcutaneous allergen‐specific immunotherapy ( AIT ). Here, we show that human basophils express CLECSF 14, DEC 205, Dectin‐1, Dectin‐2 and MRC 2. Furthermore, we demonstrate that the frequencies of basophils expressing the allergy‐associated CLR s Dectin‐1 and Dectin‐2 were significantly reduced after 1 year and 8 weeks of AIT , respectively. In contrast, the frequency of basophils positive for Fc γ RII , as well as the fraction of total basophils, significantly increased after 1 year of AIT . The herein demonstrated expression of various CLR s on basophils, and their altered CLR and FcR expression profile upon AIT , suggest yet unexplored ways by which basophils can interact with antigens and may point to novel immunoregulatory functions targeted through AIT .