Premium
Enhanced Humoral Responses Induced by Targeting of Antigen to Murine Dendritic Cells
Author(s) -
Pugholm L. H.,
Petersen L. R.,
Søndergaard E. K. L.,
Varming K.,
Agger R.
Publication year - 2015
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12387
Subject(s) - antigen , immune system , antibody , immunology , isotype , biology , monoclonal antibody , immunization , in vivo , acquired immune system , microbiology and biotechnology
Dendritic cells ( DC s) are superior in their ability to induce and control adaptive immune responses. These qualities have motivated the hypothesis that targeted delivery of antigen to DC s in vivo may be an effective way of enhancing immunization. Recent results show that antigen targeted to certain DC surface molecules may indeed induce robust immune responses. Targeting of antigen to DC s can be accomplished by the means of monoclonal antibodies. This study compared the humoral responses induced in mice by in vivo targeting of DC s using monoclonal antibodies specific for CD 11c, CD 36, CD 205, C lec6 A , C lec7 A , C lec9 A , S iglec‐ H and PDC ‐ TREM . The results demonstrate that antigen delivery to different targets on DC s in vivo gives rise to humoral responses that differ in strength. Targeting of antigen to CD 11c, CD 36, CD 205, C lec6 A , C lec7 A and PDC ‐ TREM induced significantly stronger antibody responses compared to non‐targeted isotype‐matched controls. Targeting of C lec9 A and S iglec‐ H did not lead to efficient antibody responses, which may be due to unfavourable properties of the targeting antibody, in which case, other antibodies with the same specificity might elicit a different outcome. Anti‐ CD 11c was additionally used for elucidating the impact of the route of vaccination, and the results showed only minor differences between the antibody responses induced after immunization either s.c., i.v. or i.p. Altogether, these data show that targeting of different surface molecules on DC s result in very different antibody responses and that, even in the absence of adjuvants, strong humoral responses was induced.