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Brief Exercises Affect Gene Expression in Circulating Monocytes
Author(s) -
Wang D.,
Cai F.,
Ge J.,
Yin L.
Publication year - 2015
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12345
Subject(s) - kegg , biology , gene , immune system , gene expression , gene expression profiling , transcriptome , computational biology , immunology , genetics
We aimed to give a systematic hypothesis on the functions of exercise on circulating monocytes by identifying a discrete set of genes in circulating monocytes that were altered by exercise. The microarray expression profile of GSE 51835 was downloaded from gene expression omnibus ( GEO ) database for the identification of differentially expressed genes ( DEG s) using limma and affy packages in R language. Gene Ontology ( GO ) terms and Kyoto Encyclopedia of Genes and Genomes ( KEGG ) pathway analysis were performed for DEG s, followed by the construction of co‐expression network and protein–protein interaction ( PPI ) network. The top 10 nodes in PPI network were screened, and subnetwork was constructed for the key genes identification. Totally, 35 DEG s, including 2 upregulated genes and 33 downregulated genes, were identified. The enriched GO terms were mainly linked to immune response and defence response, and the enriched KEGG pathways were mainly associated with natural killer cell‐mediated cytotoxicity and graft‐versus‐host disease. Dual‐specificity phosphatase 2 ( DUSP 2 ) was identified as a key node in the co‐expression network. In the PPI network, CD 247 module ( CD 247 ), chemokine (C‐X‐C motif) receptor 4 ( CXCR 4 ), granzyme B ( GZMB ) and perforin 1 ( PRF 1 ) were identified as key nodes. An important interaction, GZMB / PRF 1, was detected. Five key genes, including DUSP 2 , CD 247 , CXCR 4 , GZMB and PRF 1, and an interaction of GZMB / PRF 1, were significant factors in the immune processes of circulating monocytes, which might be regulated by brief exercises, leading to the enhancement of immune function.