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Association of Inducible T Cell Costimulator Polymorphisms with Susceptibility and Outcome of Hepatitis B Virus Infection in a Chinese Han Population
Author(s) -
Hu J.,
Li Q.L.,
Hou S.H.,
Peng H.,
Guo J.J.
Publication year - 2015
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12319
Subject(s) - single nucleotide polymorphism , hepatitis b virus , hepatocellular carcinoma , genotype , genotyping , medicine , snp , minor allele frequency , haplotype , population , immunology , virology , allele frequency , biology , virus , genetics , gene , environmental health
Inducible T cell costimulator ( ICOS ) functions to regulate cell–cell signalling, immune responses and cell proliferation. ICOS single nucleotide polymorphism ( SNP ) may affect protein expression and functions. This study investigated the association of ICOS SNP s with hepatitis B virus ( HBV ) infection and outcome in a Chinese population. A total of 1290 Chinese Han individuals were enrolled, including 63 asymptomatic HBV carriers, 220 chronic hepatitis B patients ( CHB ), 249 HBV ‐related liver cirrhosis patients ( LC ), 108 patients with HBV ‐related hepatocellular carcinoma ( HCC ), 338 patients with natural HBV clearance and 312 healthy subjects (as controls). DNA samples from these subjects were genotyped for four ICOS SNP s (rs11883722, rs10932029, rs1559931 and rs4675379) using TaqMan SNP Genotyping Assay and analysed. The data showed that genotype and allele frequencies of ICOS SNP s in cases and controls followed the Hardy–Weinberg distribution. The CC genotype of rs4675379 was higher in patients with HBV infection (including AC , CHB , LC and HCC ) than in patients with HBV clearance ( P = 0.006). Furthermore, the genotype ‘ GA ’ and the minor allele ‘A’ of rs1559931 were associated with a decreased HCC susceptibility ( P < 0.001). Haplotype analysis data showed that ‘ GC ’ haplotype in block 2 (rs1559931 and rs4675379) had a lower frequency in patients than in HBV ‐cleared subjects ( P = 0.034), although its overall frequency was only 1.6%. Our study found that ICOS rs1559931 SNP was associated with decreased HBV ‐related HCC risk in the studied Chinese Han population, except for patients with natural clearance of HBV .