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Haemodialysis in Diabetic Patients Modulates Inflammatory Cytokine Profile and T Cell Activation Status
Author(s) -
Almeida A.,
Lourenço O.,
Fonseca A. M.
Publication year - 2015
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12309
Subject(s) - cytokine , medicine , immunology , diabetes mellitus , inflammation , endocrinology
Diabetic nephropathy ( DN ) is a common complication in patients with diabetes, and most of them need renal replacement therapy such as haemodialysis ( HD ). These patients have a high tendency to develop infections and exhibit anomalies in the immune system. The objective of this study was to assess the expression of activation‐related markers on T cells, as well as to quantify inflammatory cytokines, before and after a single HD session in DN patients. The study involved DN patients under HD treatment who signed an informed consent form. Blood samples before and after one HD session were collected, to analyse the expression of CD 25, CD 69 and CD 71 in T cells. We also quantified IL ‐12p70, IL ‐8, IL ‐10, IL ‐1 β , TNF ‐ α and IL ‐6 in serum samples using the cytometric bead array technique. After the HD session, there was an increase in the CD 4/ CD 8 ratio due to significant alterations in both subsets. The relative percentage of CD 25+ cells and CD 8+ CD 25+ increased significantly after the HD session, while the relative percentage of CD 69 T cells decreased. There was a significant decrease in the CD 25 mean fluorescence intensity values for CD 4+ T, as well as in the case of CD 71 in T cells after the HD session. Regarding cytokine synthesis, we found a significant increase in IL ‐10 and IL ‐6 and a decrease in IL ‐8 after HD session. This study showed that a HD session in DN patients affects the T cell activation status in the two major subpopulations and differentially modulates the production of inflammatory cytokines.