Characterization of Complementary Determinant Region 3 δ in Human MutS Homologue 2‐Specific γδ T Cells

γδ T cells function as sentinels in early host responses to infections and malignancies. Previously, we found ectopically expressed human MutS homologue 2 ( hMSH 2), recognized by γδ T cells, triggered a γδ T cell‐mediated cytolysis to tumor cells. However, the characteristics of hMSH 2‐specific γδ Τ cells are not fully understood. In this study, we investigated the complementary determinant region (CDR) 3 δ diversity of hMSH 2‐specific γδ T cells. We found that the CDR3 δ sequences of hMSH 2‐specific γδ T cells displayed limited diversity, while the length and germline gene usage showed no differences compared with whole CDR3 δ immune repertoire. There are more hydrophilic amino acids in P/N insert of hMSH 2‐specific γδ T cells including the more conserved amino acid at the position 97. Our results offer clues to understanding antigen recognition pattern of γδ T cells to stress‐induced hMSH 2 of tumor cells and also the mechanism of γδ T cell‐mediated tumor immune surveillance.