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Characterization of Complementary Determinant Region 3 δ in Human MutS Homologue 2‐Specific γδ T Cells
Author(s) -
Chen H.,
Ji X.,
Cui L.,
Zhang J.,
He W.
Publication year - 2015
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12256
Subject(s) - biology , cytolysis , immune system , gene , germline , t cell , antigen , microbiology and biotechnology , function (biology) , genetics , cytotoxic t cell , in vitro
γδ T cells function as sentinels in early host responses to infections and malignancies. Previously, we found ectopically expressed human MutS homologue 2 ( hMSH 2), recognized by γδ T cells, triggered a γδ T cell‐mediated cytolysis to tumor cells. However, the characteristics of hMSH 2‐specific γδ Τ cells are not fully understood. In this study, we investigated the complementary determinant region (CDR) 3 δ diversity of hMSH 2‐specific γδ T cells. We found that the CDR3 δ sequences of hMSH 2‐specific γδ T cells displayed limited diversity, while the length and germline gene usage showed no differences compared with whole CDR3 δ immune repertoire. There are more hydrophilic amino acids in P/N insert of hMSH 2‐specific γδ T cells including the more conserved amino acid at the position 97. Our results offer clues to understanding antigen recognition pattern of γδ T cells to stress‐induced hMSH 2 of tumor cells and also the mechanism of γδ T cell‐mediated tumor immune surveillance.