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Surface‐Mediated Priming During In Vitro Generation of Monocyte‐Derived Dendritic Cells
Author(s) -
Sauter A.,
Mc Duffie Y.,
Boehm H.,
Martinez A.,
Spatz J. P.,
Appel S.
Publication year - 2015
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12246
Subject(s) - priming (agriculture) , cytokine , cd86 , ex vivo , cd14 , flow cytometry , dendritic cell , microbiology and biotechnology , dc sign , monocyte , cd38 , immunology , chemistry , cd80 , in vitro , biology , immune system , t cell , cd40 , cytotoxic t cell , cd34 , biochemistry , stem cell , botany , germination
Ex vivo ‐generated human dendritic cells ( DC ) are most commonly generated from monocytes using standard cell culture dishes. To elucidate the effect of the plastic surface during the differentiation process, we compared a standard adhesive plastic dish with four different mainly non‐adherent surfaces. Untouched monocytes were cultured for 3 days in the presence of IL ‐4 and GM ‐ CSF . Time‐lapse videos were recorded, and the phenotype of the cells was analysed by flow cytometry. The cytokine profiles were analysed using a 25‐plex cytokine assay. The use of non‐adherent surfaces led to a significant reduction in expression of CD 14 and CD 38, and a significant increase in expression of CD 86 compared to standard culture dishes. Expression levels of DC ‐ SIGN and PD ‐ L 2 were reduced significantly on cells cultured on non‐adherent surfaces. The cytokine production was independent on the surface used. The surface‐mediated priming should therefore be considered when aiming to induce specific immune responses. This is especially important with regard to DC ‐based immunotherapy, where an adjustment of the surface during the DC generation process might have highly beneficial effects.