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Expression of the T Cell‐specific Adapter Protein in Human Tissues
Author(s) -
Pandya A. D.,
Leergaard T. B.,
Dissen E.,
Haraldsen G.,
Spurkland A.
Publication year - 2014
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12199
Subject(s) - immunostaining , biology , immunohistochemistry , microbiology and biotechnology , epitope , lymphatic system , immunology , antibody
Abstract T cell‐specific adapter protein ( TSA d) encoded by the SH 2D2A gene is expressed in activated T cells, NK cells and endothelial cells, but its tissue expression has not yet been mapped. Here, we have defined the specificity of two commercially available anti‐ TSA d monoclonal reagents using peptide arrays. We found them to bind separate epitopes in the C‐terminal part of TSA d. We then used immunohistochemistry to examine TSA d expression in various human lymphoid and non‐lymphoid tissues. Immunostaining of adjacent tissue sections revealed that a substantial fraction of CD 3‐positive cells in normal lymphoid and non‐lymphoid tissues expressed TSA d. In particular, essentially all intra‐epithelial T cells appeared to coexpress TSA d. In addition, TSA d expression was observed in endothelial cells of dermal microvessels, while it was not detected in endothelial cells of the other tested tissues. This work provides insight into the expression pattern of TSA d in various healthy human tissues.