IFN γ and Perforin Cooperate to Control Infection and Prevent Fatal Pathology During Persistent Gammaherpesvirus Infection in Mice

Infection with murine gammaherpesvirus 68 has become an accepted model for studying the virus/host interactions with regard to gammaherpesvirus infections. Previous studies using gene‐deficient mice have revealed that neither IFN γ nor perforin is essential in controlling the outcome of infection or the virus load during chronic infection in C57BL/6 mice. However, pronounced multiorgan fibrosis and splenic atrophy are observed in mice lacking IFN γ or the IFN γ receptor. To study the interplay between perforin and IFN γ in controlling the virus‐induced pathology and the viral load during chronic gammaherpesvirus infection, we infected IFN γ /perforin double‐deficient C57BL/6 mice and followed the course of infection. While absence of perforin prevented the splenic atrophy in IFN γ ‐deficient mice, fibrosis did not disappear. Moreover, double‐deficient mice developed extreme splenomegaly, were unable to control the viral load and displayed chronic immune activation. Thus, IFN γ and perforin act in concert to minimize pathology and control the viral load in mice chronically infected with MHV 68. Furthermore, while certain aspect of the virus‐induced pathology in IFN γ ‐deficient mice may be alleviated in double‐deficient mice, other aspects are exaggerated, and the normal architecture of the spleen is completely destroyed. We believe that these findings add to the understanding of the virus/host interaction during chronic gammaherpes virus infection.