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The Lymphoid Chemokine CCL 21 Enhances the Cytotoxic T Lymphocyte‐Inducing Functions of Dendritic Cells
Author(s) -
Hong C. Y.,
Lee H.J.,
Kim H.J.,
Lee J.J
Publication year - 2014
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12145
Subject(s) - ctl* , cytotoxic t cell , chemokine , immunology , dendritic cell , cancer research , chemistry , biology , immune system , in vitro , biochemistry
The potential use of lymphoid chemokines to generate a dendritic cell ( DC ) cancer vaccine is not yet clear. We investigated the effect of lymphoid chemokines on DC function and on the production of effective cytotoxic T lymphocytes ( CTL s) for application of cancer vaccine using monocyte‐derived mature DC s ( mDC s) prestimulated with lymphoid chemokines. mDC s exposed to a secondary lymphoid organ chemokine ( SLC / CCL 21) dramatically induced CTL response by increasing cytolytic activity without any significant alterations on expression of cell surface markers (e.g. CD 80, CD 83, CD 86 and CCR 7) or on the production of cytokines (e.g. IL ‐12p70, IL ‐10 and IL ‐23). Interestingly, mDC s prestimulated with CCL 21 showed higher levels of CXCL 10 ( IP ‐10) production, but not the production of CCL 22, compared with untreated mDC s. IP ‐10 treatment during CTL generation with DC s dramatically enhanced tumour‐specific CTL response compared with untreated CTL s, and these enhanced CTL ‐inducing functions of CCL 21‐treated DC s were inhibited by anti‐ IP ‐10 treatment. Taken together, our data suggested an important role of the lymphoid‐endothelium‐associated chemokine, CCL 21, on DC s in the induction of CTL responses.