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Upregulation of Membrane‐Bound CD 40L on CD 4 + T cells in Women with Primary Sjögren's Syndrome
Author(s) -
Belkhir R.,
Gestermann N.,
Koutero M.,
Seror R.,
Tost J.,
Mariette X.,
MiceliRichard C.
Publication year - 2014
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12121
Subject(s) - cd40 , epigenetics , demethylating agent , microbiology and biotechnology , downregulation and upregulation , methylation , dna methylation , immunology , demethylation , biology , chemistry , endocrinology , gene expression , gene , genetics , cytotoxic t cell , in vitro
Epigenetic deregulation of genes encoded on the X chromosome as reported for CD 40L in lupus could explain the female predominance of autoimmune diseases. We compared CD 40L expression on CD 4 + T cells from primary Sjögren's syndrome (p SS ) women and healthy controls and investigated DNA methylation patterns of the promoter and enhancer regions of CD 40L . The expression of CD 40L on activated CD 4 + T cells was higher in patients with p SS than controls after phorbolmyristate acetate and ionomycin activation ( P = 0.02). CD40L mRNA level in CD 4 + T cells did not differ between patients with pSS and controls and was similar in both groups in cultures treated with the demethylating agent 5‐azacytidine C. Pyrosequencing analysis revealed no significant differences in methylation profiles between patients and controls. Inducible membrane‐bound CD40L on CD4 + T cells is increased in patients with pSS but was not related to epigenetic deregulation by demethylation patterns of the regulatory regions of CD40L .