Premium
Cultured Mast Cells from Patients with Asthma and Controls Respond with Similar Sensitivity to Recombinant D er P 2‐Induced, I g E ‐Mediated Activation
Author(s) -
Krohn I. K.,
Sverrild A.,
Lund G.,
Dahl R.,
Erjefält J. S.,
Backer V.,
Hoffmann H. J.
Publication year - 2013
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12085
Subject(s) - asthma , allergen , atopy , immunology , recombinant dna , allergy , medicine , mast cell , chemistry , biochemistry , gene
The function of cultured mast cells may depend on genetic or environmental influence on the stem cell donor. This study investigates whether asthma or atopy in the donor influenced the growth and sensitivity of mast cells cultured from patients with asthma and healthy controls under identical conditions. Mast cells were cultured from peripheral blood from twelve patients with an objectively confirmed asthma diagnosis and eight healthy subjects. During the last 2 weeks of culture, mast cells were incubated with IL ‐4 and 80 kU/l recombinant human I g E containing two clones (7% + 7%) specific for mite allergen D er p2. The sensitivity of I g E ‐mediated activation of mast cells was investigated as F cε RI ‐mediated upregulation of CD 63. Ten subjects were atopic, defined as a positive skin prick test (>3 mm) to at least one of ten common allergens. After activation with recombinant D er p2, the maximum CD 63 median fluorescence intensity was 20 456 ± 1640 ( SE ) for patients with asthma and 22 275 ± 1971 ( SE ) for controls (ns). The fraction of CD 63 positive cells was 54.4% in patients with asthma and 48.4% in controls (ns). The allergen concentration inducing 50% of the maximal CD 63 response was similar in patients with asthma [−0.4795 log ng/ml ± 0.092 ( SE )] and controls (−0.6351 log ng/ml ± 0.083, ns) and in atopic and non‐atopic subjects. When cultured, sensitized and activated under identical conditions, mast cells from allergic asthmatics and healthy controls respond similar. Activation of cultured mast cells appears to depend on culture conditions ( IL ‐4, I g E ) rather than on donor status as atopy and asthma.