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Involvement of RP 105 and Toll‐Like Receptors in the Activation of Mouse Peritoneal Macrophages by Staphylococcus aureus
Author(s) -
Liu B.,
Fu Y.,
Feng S.,
Zhang X.,
Liu Z.,
Cao Y.,
Li D.,
Liang D.,
Li F.,
Zhang N.,
Yang Z.
Publication year - 2013
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12050
Subject(s) - tlr2 , staphylococcus aureus , tlr4 , chemokine , toll like receptor , microbiology and biotechnology , immunology , receptor , biology , immune system , innate immune system , bacteria , genetics , biochemistry
Staphylococcus aureus is the aetiological agent of many hospital‐ and community‐acquired infections. Toll‐like receptor 2 ( TLR 2) has been shown to play a crucial role in the host defence against S. aureus infection. The aim of this study is to investigate the roles of the heterogeneous TLR family proteins TLR 2, TLR 4 and RP 105 during S. aureus infection. Peritoneal macrophages from mice were exposed to S. aureus . Their production of inflammatory cytokines and chemokines, their expression of cell‐surface markers and interactions between TLR 2, TLR 4 and RP 105 were assessed in the presence or absence of inhibitory antibodies against TLR 2, TLR 4/ MD ‐2 and RP 105/ MD ‐1 complexes. Our results demonstrate that not only TLR 2 but also TLR 4 and RP 105 are involved in the response of macrophages to S. aureus , that TLR 2, TLR 4 and RP 105 physically interact with each other during S. aureus infection, and that TLR 2, TLR 4 and RP 105 both cooperate and play unique roles in the production of inflammatory cytokines ( TNF ‐α, IL ‐12p40 and IL ‐10) and chemokine ( RANTES ) by macrophages after S. aureus infection. This study characterizes the important roles that TLR 2, TLR 4 and RP 105 play in host resistance against S. aureus infection.