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Quinolinic Alkaloids from G alipea longiflora K rause Suppress Production of Proinflammatory Cytokines in vitro and Control Inflammation in vivo upon L eishmania Infection in Mice
Author(s) -
CallaMagariños J.,
Quispe T.,
Giménez A.,
Freysdottir J.,
TroyeBlomberg M.,
Fernández C.
Publication year - 2013
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12010
Subject(s) - in vivo , proinflammatory cytokine , meglumine antimoniate , spleen , in vitro , pharmacology , berberine , immunology , inflammation , biology , chemistry , medicine , visceral leishmaniasis , leishmaniasis , biochemistry , microbiology and biotechnology
An antileishmanial activity of quinolinic alkaloids from G alipea longiflora Krause , known as Evanta, has been demonstrated. We have previously shown that, apart from its leishmanicidal effect, in vitro pretreatment of spleen cells with an alkaloid extract of Evanta ( AEE ) interfered with the proliferation and interferon‐γ production in lymphocytes polyclonally activated either with concanavalin A or anti‐ CD 3. In the present study, we investigated if AEE could interfere with antigen‐specific lymphocyte activation. We found that in vitro and in vivo treatment reduced recall lymphocyte responses, as measured by IFN ‐γ production (55% and 63% reduction compared to untreated cells, respectively). Apart from IFN ‐γ, the production of IL ‐12 and TNF was also suppressed. No effects were observed for meglumine antimoniate ( S b V ), the conventional drug used to treat leishmaniasis. When mice infected with L eishmania braziliensis promastigotes in the hind footpad were treated with AEE , the dynamics of the infection changed and the footpath thickness was efficiently controlled. The parasite load was also reduced but to a lesser extent than upon treatment with S b V . Combined treatment efficiently controlled both the thickness and parasite load as smaller lesions during the entire course of the infection were seen in the mice treated with AEE plus S b V compared with AEE or S b V alone. We discuss the benefits of combined administration of AEE plus S b V .