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The Ex Vivo Induction of Human CD 103 + CD 25 hi Foxp3 + CD 4 + and CD 8 + Tregs is IL ‐2 and TGF ‐β1 Dependent
Author(s) -
Gunnlaugsdottir B.,
Maggadottir S. M.,
Skaftadottir I.,
Ludviksson B. R.
Publication year - 2013
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/sji.12009
Subject(s) - ex vivo , chemistry , biochemistry , in vitro
The expression of the integrin αE ( CD 103), may enhance the retention of regulatory T cells to peripheral inflammatory sites and possibly contribute to their suppressive potential. The aim of this study was to define the regulatory role of IL ‐2 and TGF ‐β1 on the CD 103 expression and the optimal in vitro conditions for the induction/expansion of human CD 4 + and CD 8 + Tregs. Cord blood mononuclear cells ( CBMC ) were stimulated under various culture conditions, including anti‐ CD 3, anti‐ CD 28, IL ‐2 and TGF ‐β1. TGF ‐β1 and IL ‐2 were both required for optimal expression of CD 103. In addition, TGF ‐β1 and IL ‐2 synergistically induced CD 103 expression on CD 8 + T cells, whereas, only additive induced expression was noted on CD 4 + T cells. Surprisingly, CD 103 expression was not dependent upon CD 28 costimulation. IL ‐2 also played a central role in CD 103 expression by CD 25 hi F oxp3+ Tregs. IL ‐2, TGF ‐β1 and anti‐ CD 3 defined the optimal stimulatory conditions favouring the induction/expansion of both CD 4 + and CD 8 + human Tregs from naive CBMC . Thus, this study provides new insights into the regulatory role of IL ‐2 upon CD 103 expression by human cord blood CD 4 + and CD 8 + T cells. Furthermore, it identifies the in vitro culture conditions driving the differentiation of the novel phenotype CD 4 + and CD 8 + CD 103 + CD 25 hi Foxp3+ Tregs from human CBMC .