Premium
Evaluation of DEFB1 polymorphisms in individuals with chronic periodontitis and diabetes mellitus type 2 in a population of northeastern Brazil
Author(s) -
Soraia Aguiar de Melo Dias Rayanne,
Carlos Mourão Pinho Roberto,
Almeida Felipe Rodrigues de,
Alfredo Farias Bandeira Francisco,
Celerino da Silva Ronaldo,
Crovella Sergio,
Carvalho Farias Vajgel Bruna,
Cimões Renata
Publication year - 2018
Publication title -
special care in dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.328
H-Index - 41
eISSN - 1754-4505
pISSN - 0275-1879
DOI - 10.1111/scd.12296
Subject(s) - single nucleotide polymorphism , haplotype , allele , genotype , snp , medicine , context (archaeology) , genetics , periodontitis , chronic periodontitis , polymorphism (computer science) , biology , gene , paleontology
Aims The role of genetic variations in genes related to innate response, as β‐defensin‐1 ( DEFB1 ), in the context of chronic periodontitis (CP) and diabetes mellitus type 2 (DM2), is still not clear. The present study evaluates the distribution of DEFB1 single nucleotide polymorphisms (SNPs) 5′‐untranslated (5′UTR) region and its relation with the CP in DM2 individuals in northeastern Brazilians. Methods Two hundred and eighty individuals participated in the study, being 116 DM2+CP, 95 CP, and 69 healthy individuals. Three known DEFB1 functional SNPs [‐52 G > A (rs1799946), ‐44 C > G (rs1800972), ‐20 G > A (rs11362)] were genotyped with allele‐specific assays. Results Association was found for the DEFB1 ‐20 G > A SNP. The G allele, the GA and GG genotypes were significantly ( P < 0.05) more frequent in the DM2+CP (59.5%, 50%, and 34.5%, respectively) and CP (61%, 44.2%, and 38.9%, respectively) than in healthy individuals (26.8%, 36.2%, and 8.7%, respectively). The GCG and ACG combinations (‐52, ‐44, ‐20) were significantly more frequent among DM2+CP and CP than in the healthy individuals. Conclusion The results indicate that genetic variations of DEFB1 gene (SNP‐20: G allele and GA and GG genotypes) and the DEFB1 5′UTR haplotypes (GCG and ACG) may be associated with a susceptibility to CP in DM2 individuals as well as CP individuals without DM2.