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Reduced salivary flow rate and high levels of oxidative stress in whole saliva of children with Down syndrome
Author(s) -
Domingues Natália Bertolo,
Mariusso Matheus Racy,
Tanaka Marcia Hiromi,
ScarelCaminaga Raquel Mantuaneli,
Mayer Marcia Pinto Alves,
Brighenti Fernanda Lourenção,
Zua Ângela Cristina Cilense,
Ibuki Flávia Kazue,
Nogueira Fernando Neves,
Giro Elisa Maria Aparecida
Publication year - 2017
Publication title -
special care in dentistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.328
H-Index - 41
eISSN - 1754-4505
pISSN - 0275-1879
DOI - 10.1111/scd.12258
Subject(s) - malondialdehyde , saliva , superoxide dismutase , oxidative stress , medicine , antioxidant , endocrinology , gastroenterology , dentistry , biochemistry , chemistry
ABSTRACT Aim To investigate salivary parameters between children with Down Syndrome (DS) and without DS. Materials and Methods Stimulated whole saliva was collected from 18 children with DS and 23 without DS. Salivary flow rate, pH, and salivary buffering capacity were determined. Cariogenic microorganisms were quantified by culture, and periodontopathogens by quantitative real‐time polymerase chain reaction. The antioxidant profile was quantified spectrophotometrically, while malondialdehyde (MDA) was quantified by high‐performance liquid chromatography. Data were analyzed by Mann‐Whitney test and Spearman correlation (α = 0.05). Results Salivary flow rate was significantly lower in DS than in controls ( p < 0.0001). Significant higher difference was observed for total protein dosage ( p < 0.0001), superoxide dismutase activity (SOD) ( p = 0.0002), and MDA ( p < 0.001) in DS group. Conclusions Reduced salivary flow rate might be an important factor in oral diseases development. High salivary levels of SOD and MDA show the significant influence of the oxidative stress and the early‐onset periodontal disease in DS people.