Premium
Comparison of in vivo probe‐based confocal laser endomicroscopy with histopathology in lung cancer: A move toward optical biopsy
Author(s) -
Wellikoff Adam S.,
Holladay Robert C.,
Downie Gordon H.,
Chaudoir Catherine S.,
Brandi Luis,
TurbatHerrera Elba A.
Publication year - 2015
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/resp.12578
Subject(s) - medicine , histopathology , endomicroscopy , lung cancer , pathology , biopsy , radiology , lung , confocal , geometry , mathematics
Background and objective The development of novel technologies has increased the yield from transbronchial biopsies while preserving patient safety by guiding biopsies to the area of interest. Other technologies have helped identify pre‐cancerous or sessile lesions in the endobronchial space by utilizing interactions between tissue and light at varying wavelengths. Probe‐based confocal laser endomicroscopy ( pCLE ) is a new technology that encompasses the benefits of both guided biopsies and novel optical imaging in one device. This project compares pCLE images to the findings of light microscopy in non‐small cell lung cancer ( NSCLC ). Methods Patients who underwent bronchoscopies between J uly 2012 and J anuary 2013 for evaluation of pulmonary lesions (transbronchial and endobronchial) were recruited. Histopathological images from malignant lesions were compared with the pCLE images obtained from the same area. The microscopic and pCLE images were reviewed side by side with the microscopic findings. Results Images from pCLE correlate with some histopathological findings. pCLE changes seen in NSCLC consist of mottled elastin, septal studding and disorganization/fragmentation with increased friability. These changes also seem to correlate with degrees of differentiation. Conclusions pCLE can identify changes to the elastin composition of the airways and alveoli in lung cancer. These changes correlate with histopathology and may help indicate the presence of malignant changes in vivo.