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Possible role of anaerobes in the pathogenesis of nontuberculous mycobacterial infection
Author(s) -
Yamasaki Kei,
Mukae Hiroshi,
Kawanami Toshinori,
Fukuda Kazumasa,
Noguchi Shingo,
Akata Kentarou,
Naito Keisuke,
Oda Keishi,
Ogoshi Takaaki,
Nishida Chinatsu,
Orihashi Takeshi,
Kawanami Yukiko,
Ishimoto Hiroshi,
Taniguchi Hatsumi,
Yatera Kazuhiro
Publication year - 2015
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/resp.12536
Subject(s) - nontuberculous mycobacteria , medicine , microbiology and biotechnology , pathogenesis , mycobacterium avium complex , immunology , tuberculosis , mycobacterium , pathology , biology
Background and objective Recent advances in cultivation‐independent molecular biological modalities for detecting bacterial species have indicated that several bacterial species may play a role in the pathogenesis of certain infectious diseases. The aim of this study was to evaluate the role of bacterial flora in the pathogenesis of nontuberculous mycobacteriosis ( NTM ) using a bacterial floral analysis of bronchoalveolar lavage fluid ( BALF ) with 16S rRNA gene sequencing in patients with bronchiectasis. Methods Fifty‐eight patients with bronchiectasis evaluated using chest computed tomography were enrolled. BALF obtained from the most affected lung lesions was evaluated using culture and culture‐independent methodologies. Approximately 600 bp of the bacterial 16S rRNA gene ( E341F‐E907R ) was amplified via polymerase chain reaction using universal primers, and clone libraries were constructed. Nucleotide sequences of 96 randomly chosen clones for each specimen were determined, and the homology was searched using a basic local alignment search tool to determine the bacterial phylotypes and their proportions (bacterial floral analysis) in each specimen. Results Twenty‐nine patients with bronchiectasis were diagnosed with NTM based on culture‐based methods using Ogawa medium. The molecular method showed a significantly high rate of anaerobes among the patients with NTM compared with that observed in the bronchiectasis patients without NTM . In addition, findings of collapse/consolidation were significantly related to the proportion of Prevotella species in the BALF samples determined using the molecular method ( P < 0.001). Conclusion Given the results of the present study, anaerobes may play an important role in the pathogenesis of bronchiectasis in patients with NTM .