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Paraneoplastic H u and CRMP5 antibodies are present in smokers without cancer or neurological disease
Author(s) -
Qvale Tor H.,
Storstein Anette,
Mazengia Kibret,
Eagan Tomas M.L.,
Bakke Per S.,
Vedeler Christian A.
Publication year - 2014
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/resp.12292
Subject(s) - medicine , disease , cancer , antibody , lung cancer , immunology
Background and objective We investigated if the paraneoplastic H u and collapsin response mediator protein 5 (CRMP5) antibodies could be used as early markers for lung cancer in smokers with or without chronic obstructive pulmonary disease ( COPD ). Methods H u and CRMP5 antibodies were measured by radioimmunoprecipitation assay ( RIPA ) in sera from 552 smokers; 379 with and 173 without COPD . Three hundred blood donors served as controls. The positive sera were also tested by indirect immunofluorescence and line blot with recombinant proteins. The 552 smokers were matched with data from the C ancer R egistry of N orway, and the hospital medical records from the subjects positive for H u and CRMP5 antibodies were reviewed. The mean follow‐up time was 4.4 years (range 2.5–5.7 years). Results The RIPA showed that 5/379 (1.3%) smokers with COPD had H u antibodies and 1/379 (0.3%) smokers with COPD had CRMP5 antibodies. Only the smoker with the highest RIPA index had H u antibodies also detected by immunofluorescence and line blot. One of 173 (0.6%) smokers without COPD had H u antibodies, but none had CRMP5 antibodies. None of the 300 controls had H u antibodies, but 2/300 (0.7%) had CRMP5 antibodies. H u antibodies remained positive for more than 5 years. No cancer or neurological disease was recorded in the H u or CRMP5 positive patients. The total cancer frequency in the smokers with and without COPD was 70/552 (13%). Conclusions H u and CRMP5 antibodies were not associated with cancer or neurological disease in a large cohort of smokers and are therefore not always paraneoplastic.