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D er p2 recombinant bacille C almette‐ G uerin priming of bone marrow‐derived dendritic cells suppresses D er p2‐induced T helper17 function in a mouse model of asthma
Author(s) -
Qu ShuoYao,
Ouyang HaiFeng,
He YaLong,
Li ZhiKui,
Shi JieRan,
Song LiQiang,
Wu ChangGui
Publication year - 2014
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/resp.12198
Subject(s) - priming (agriculture) , dendritic cell , adoptive cell transfer , inflammation , t cell , allergic inflammation , immunology , microbiology and biotechnology , cellular differentiation , immune system , reprogramming , medicine , biology , cell , biochemistry , botany , germination , gene
Background and objective Previous studies have demonstrated that our recombinant bacille C almette‐ G uerin ( rBCG ), which expresses D er p2 in house dust mite ( D er p2 rBCG ) suppresses asthmatic airway inflammation by regulating the phenotype and function of dendritic cells ( DC ) and reprogramming T helper ( T h) 0 cell differentiation into different T cell ( T h1/ T h2/ T reg) subtypes. However, the exact role of D er p2 rBCG in reprogramming T h17 differentiation and the relevant mechanisms are not known. The aim of this study was to examine whether D er p2 rBCG ‐mediated inhibition of allergic airway inflammation is mediated by regulating T h17 differentiation in a murine asthma model. Methods Primary mouse bone marrow‐derived dendritic cells ( BMDC ) were infected with D er p2 rBCG and adoptively transferred to D er p2‐intranasally sensitized mice. The role of D er p2 rBCG ‐ BMDC on the regulation of airway inflammation and T h17 cell differentiation was assessed. Results Adoptive transfer of D er p2 rBCG ‐ BMDC suppressed airway inflammation and mucin secretion. D er p2 rBCG ‐ BMDC inhibited excessive T h17 immune responses but not BCG ‐ BMDC . Furthermore, D er p2 rBCG decreased jagged‐2 and increased delta‐like‐4 expressions on BMDC to a greater extent than BCG . Conclusions These findings suggest that DC plays a key role in D er p2 rBCG ‐induced immunoregulation. D er p2 rBCG also displayed a potent inhibitory effect on T h17 differentiation, and these findings increase our understanding of the cellular basis of D er p2 BCG ‐mediated inhibition of asthma.