Premium
Aminopeptidase N facilitates entry and intracellular survival of M ycobacterium tuberculosis in monocytes
Author(s) -
Ho HsinTsung,
Tsai IFang,
Wu ChienLiang,
Lu YenTa
Publication year - 2014
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/resp.12191
Subject(s) - intracellular , internalization , mycobacterium tuberculosis , flow cytometry , tuberculosis , medicine , receptor , microbiology and biotechnology , immunology , biology , pathology
Background and objective Aminopeptidase N ( CD13 ) is an ectoenzyme located in the outer membrane of a variety of cells. Proteomic profiling indicates an increased expression of CD13 in phagocytes during M ycobacterium tuberculosis infection. The purpose of this study was to investigate the role of CD13 on the internalization and intracellular survival of M . tuberculosis in monocytes. Methods Magnetic nanoparticles and confocal microscopy were used to observe interactions between CD13 and M . tuberculosis . Mycobacterial entry and intracellular survival in monocytes were assessed with and without anti‐ CD13 antibody ( WM15 and WM47 ) using flow cytometry and colony formation assay. Results By using magnetic nanoparticles and confocal microscopy, M . tuberculosis was found to be capable of binding to either soluble CD13 or membranous CD13 on monocytes. Flow cytometry showed that pretreatment of monocytes with WM15 or WM47 reduced the number of intracellular M . tuberculosis . Collectively, the data suggest that CD13 is a binding and entry receptor for M . tuberculosis on monocytes. Treatment of infected monocytes showed a greater effect of WM47 than WM15 in reducing the intracellular colonization of M . tuberculosis , suggesting that specific epitopes of CD13 may play an important role modulating intracellular M . tuberculosis survival. Conclusions CD13 acts as a receptor for M . tuberculosis on human monocytes. The molecule facilitates internalization, and interaction of CD13 with an anti‐ CD13 antibody reduces intracellular M . tuberculosis survival.