Premium
Respiratory syncytial virus induces leukotriene C 4 synthase expression in bronchial epithelial cells
Author(s) -
Sun Lihong,
Chen Aihuan,
Yang Zifeng,
Chen Jason J.,
Guan Wenda,
Wu Jieling,
Qin Sheng,
Zhong Nanshan
Publication year - 2013
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/resp.12188
Subject(s) - medicine , respiratory system , leukotriene c4 , virus , leukotriene , atp synthase , immunology , virology , enzyme , asthma , biochemistry , biology
Background and objective Respiratory syncytial virus ( RSV ) results in acute wheezing in infants and is frequently associated with recurrent wheezing. Although RSV ‐induced wheezing clinically resembles that of asthma, corticosteroids are not equivalently effective in RSV ‐associated wheezing. The study sought to determine the mechanisms of RSV ‐induced wheezing by establishing an in vitro model of RSV ‐infected human bronchial epithelial cells (16‐ HBEC ). Methods Leukotriene C 4 synthase ( LTC 4 S ) messenger RNA ( mRNA ) expression in 16‐ HBEC was detected using fluorescence quantitative polymerase chain reaction, and the relative level of LTC 4 S mRNA was expressed as quotient cycle threshold ( qCt ) based on the threshold cycle number value compared with that of β‐actin. Cysteinyl leukotrienes ( CysLT ) in culture supernatant were measured by enzyme‐linked immunosorbent assay. RSV ‐infected 16‐ HBEC was incubated with gradient concentration of budesonide ( BUD ) to assess its effects on LTC 4 S expression and CysLT secretion. Results RSV infection resulted in increased LTC 4 S mRNA expression between 48 and 96 h post‐infection. High level of CysLT was detected in the supernatant of RSV ‐infected 16‐ HBEC . BUD at concentrations of 10 −10 to 10 −5 mol/ L did not significantly alter LTC 4 S mRNA expression. Conclusions RSV infection upregulated LTC 4 S expression in HBEC leading to increased CysLT secretion. Such induction was not attenuated by BUD , suggesting that CysLT might contribute to the pathogenesis of RSV ‐induced wheezing.