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Alterations in inflammatory, antiviral and regulatory cytokine responses in peripheral blood mononuclear cells from pregnant women with asthma
Author(s) -
Vanders Rebecca L.,
Gibson Peter G.,
Wark Peter A. B.,
Murphy Vanessa E.
Publication year - 2013
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1111/resp.12068
Subject(s) - phytohaemagglutinin , medicine , immunology , peripheral blood mononuclear cell , pregnancy , asthma , immune system , in vitro , biology , biochemistry , genetics
Background & objective Severe asthma exacerbations during pregnancy are a common complication leading to poor health outcomes for both the mother and the baby. Asthma exacerbations are caused most frequently by respiratory viruses. A balance between antiviral and inflammatory immune responses is critical during pregnancy; the balance may be altered by asthma and respiratory virus infection. Methods Peripheral blood mononuclear cells ( PBMC s) were isolated from (i) non‐pregnant healthy controls, (ii) pregnant non‐asthmatics, (iii) post‐partum non‐asthmatics, (iv) non‐pregnant asthmatics (v) pregnant asthmatics, and (vi) post‐partum asthmatics. Cells were cultured in vitro with the mitogen phytohaemagglutinin or with a strain of the 2009 pandemic swine influenza. Interferon (IFN)‐γ , interleukin ( IL )‐10 and IL ‐17 protein were measured from culture supernatant. Neutrophil counts were obtained in samples from pregnant and post‐partum women. Results Following the phytohaemagglutinin stimulation of PBMC s, pregnant asthmatics had significantly higher IL ‐17 and significantly lower IFN ‐γ responses compared with healthy non‐pregnant women. Following infection with influenza, a significant reduction was also observed in IFN‐γ and IL‐10 production from PBMC of pregnant asthmatics. The IL‐17 response to phytohaemagglutinin correlated with the neutrophil percentage. Differences in IFN‐γ , IL‐10 and IL‐17 were found to persist for at least 6 months post‐partum. Conclusions A reduction in antiviral and regulatory immunity with increased inflammation during pregnancy occurs in PBMC from pregnant women with asthma. This novel information may relate to the increased susceptibility and disease severity to respiratory virus infections observed during pregnancy.