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Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device
Author(s) -
Brandão Fabiana Aparecida Santilli,
Alves Kele Amaral,
Brito Danielle Cristina Calado,
Pereira Leda Maria Costa,
Morais Glayciane Bezerra,
Ñaupas Lucy Vanessa Sulca,
Souza Samara Silva,
Alves Benner Geraldo,
Rodrigues Ana Paula Ribeiro,
Teixeira Dárcio Ítalo Alves
Publication year - 2021
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.13979
Subject(s) - ovarian tissue , vitrification , ovary , ovarian tissue cryopreservation , andrology , medicine , gynecology , fertility preservation , fertility , population , environmental health
Abstract The present study evaluated the effect of Ovarian Tissue Cryosystem (OTC) on follicular morphology and density, as well as on stromal cell density of vitrified canine ovarian tissue. Canine ovarian fragments collected from adult female dogs in stages of the random oestrous cycle were fixed (FC, fresh control) or vitrified (VIT) with an OTC device. After vitrification and warming, the fragments were fixed for histological analysis. Overall, the mean percentage of normal pre‐antral follicles decreased after vitrification procedure (FC: 74.5% ± 1.6% vs. VIT: 52.05% ± 1.5%). Although the rates of normal primordial (71.1% ± 1.8%) and secondary (0.7% ± 0.4%) follicles vitrified showed a reduction ( p < .05), vitrification using OTC showed considerable preservation of follicles, when compared to the fresh control (81.1% ± 1.5% and 2.3% ± 0.6%, respectively). The mean follicular density was maintained after vitrification (FC: 199.65 ± 12.8 vs. VIT: 199.68 ± 10.8), whereas the stromal cell density decreased in the VIT group. Based on the results, we recommend the use of OTC for vitrification of canine ovarian tissue.